Common antigens among systemic disease fungi analyzed by two-dimensional immunoelectrophoresis
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چکیده
منابع مشابه
Characterization and comparison of mycobacterial antigens by two-dimensional immunoelectrophoresis.
Two-dimensional immunoelectrophoresis (2D-IEP), in which a complex of antigens is subjected to electrophoresis first through an agarose matrix in one direction and secondly through an antiserum-agarose matrix at right angles to the first direction, was evaluated as a tool for analysis of mycobacterial antigens. Cell extracts from four species of mycobacteria, Mycobacterium tuberculosis (four st...
متن کاملCharacterization of membrane and cytoplasmic antigens of Mycoplasma arginini by two-dimensional (crossed) immunoelectrophoresis.
Two-dimensional immunoelectrophoresis was employed to electrophoretically identify membrane and cytoplasmic antigens of Mycoplasma arginini G-230. Five distinct cytoplasmic antigens were observed in soluble fractions prepared by digitonin lysis with electrophoretic mobilities (relative to bovine albumin) ranging from 0.36 to 0.86; four of these were common to other M. arginini strains: leonis a...
متن کاملRapid two-dimensional immunoelectrophoresis of human serum proteins.
We describe two versions of a two-dimensional immunoelectrophoretic system and illustrate their use for separating and estimating human serum proteins. Each is sensitive, rapid, and simple to perform, and requires no special apparatus and only 0.1-0.6 ml of antiserum. Both procedures appear to be potentially useful in the clinical laboratory. Time required to obtain a stained pattern varies fro...
متن کاملRocket and Two Dimensional Immunoelectrophoresis in Diagnosis of Caprine Brucellosis
BACKGROUND Brucellosis is a major bacterial zoonosis of global importance with the causative organisms of Gram-negative facultative intracellular pathogens. The aims of this study were to standardize two immunoelectrophoretic techniques, rocket and cross immunoelectrophoresis, and compare their results with other conventional serodiagnostic tests. METHODS Sera from 15 sheep, without any histo...
متن کاملModifications to the technique of two-dimensional immunoelectrophoresis.
In recent years a number of sensitive immunological techniques have been developed to study proteins in biological fluids. The most recently described is that of Minchin Clarke & Freeman (1966) which is a variant of an immunoelectrophoretic method originally described by Ressler (1960) and given the name of crossed immunoelectrophoresis by Laurel1 (1966). It is highly sensitive and by using a c...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1979
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.23.2.479-485.1979